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. 2017 Sep 25;114(41):10984–10989. doi: 10.1073/pnas.1704835114

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Fig. 3. — Catalytically active Drok rescues the ARM deficits of drk mutant heterozygotes. Mean performances ± SEM are shown. Controls are represented by black bars, mutants by open bars, and mutant animals with potential behavioral rescue by gray bars. Following ANOVA, potential differences were assessed for significance with planned comparisons (least square means contrast analyses) as necessary. (A) Adult-specific expression of the constitutively active UASDrok^CAT within α/β neurons rescued (gray bars) the ARM deficit of drk^ΔP24/+ flies (open bar). The entire data shown are from animals induced in parallel. Whereas drk^ΔP24/+; Drok^CAT/Gal80^ts flies were significantly (P < 0.0001) deficient in ARM compared with any of the controls (black bars), drk^ΔP24/c772; Drok^CAT/Gal80^ts were not (P = 0.9967). Similarly, drk^ΔP24/c739; Drok^CAT/Gal80^ts flies performed equally well with controls (P = 0.688) but significantly different (P < 0.0001) from drk^ΔP24/+; Drok^CAT/Gal80^ts animals. n > 7 for all. (B) Rescue of the ARM deficit of drk^ΔP24/+; Drok^CAT/Gal80^ts animals (open bar) by induction of an independent Drok^CAT transgene on chromosome II (gray bar). Whereas the performance of drk^ΔP24, Drok^CAT/c772; +/Gal80^ts was not different from that of controls (P = 0.829), it was significantly different from that of drk^ΔP24, Drok^CAT/+; +/Gal80^ts (P < 0.0007). (C) The catalytically inactive Drok^KG transgene does not rescue (light gray bar) the ARM deficit of drk^ΔP24, Drok^KG/+; +/Gal80^ts (P = 0.774) under the c739 driver, which recues (P = 0.731) the drk^ΔP24 deficit with the Drok^CAT transgene (drk^ΔP24, Drok^CAT/c739; +/Gal80^ts). n > 7. (D) Conditional expression of Drok^CAT in α/β neurons rescues (gray bars) the 3-h memory deficit of drk^ΔP24 heterozygotes after cold shock. Uninduced (UN) drk^ΔP24/c739; Drok^CAT/Gal80^ts animals were used as negative controls (open bars), and they performed significantly different from controls (P < 0.0002), while after induction they did not (gray bars, P = 0. 849). Similarly, ARM after cold shock was significantly different in UN (open bars) drk^ΔP24/c739; Drok^CAT/Gal80^ts from controls (^+/+; Drok^CAT/Gal80^ts, or +/c739; +/Gal80^ts; P < 0.003 for both) and from the same flies after transgene induction (gray bar; P < 0.0015). n > 7.