Fig. 6.

Cell nuclear volume and nuclear dynamics change with cell volume. (A) Nucleus volume always directly tracks cell volume, decreasing proportionally with increasing substrate stiffness, spread area, and osmotic pressure; the ratio between nuclear volume and cell volume remains approximately constant for each tested cell type. (B) Mean square displacement of GFP-tagged Histone in MCF-10A cell nuclei, reflecting positional fluctuation of chromatin, significantly reduces under external osmotic compression through application of 0.1 M PEG 300. osm. comp., osmotic compression; sub., substrate.