Fig. 3.

Effect of rapamycin and torin-1 on human TPC2 WT and G734E. (A and B) Representative PI(3,5)P₂ (1 μM) activated currents in vacuolin-enlarged hTPC2 WT and hTPC2(G734E) expressing endolysosomal vesicles and the respective blocking effects of 2 μM torin-1 or 1 μM rapamycin. Cells were incubated with torin-1 for 12 h or with rapamycin for 10 min before experimentation. Currents were measured in the absence of ATP. (C) Statistical summary of data as shown in A and B including respective basal currents. Shown are average current densities at −100 mV. To test for statistical significance the one-way ANOVA test followed by Tukey’s posttest was applied to basal and PI(3,5)P₂ conditions, respectively. ***, ^###P < 0.001, ^##P < 0.01.