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. 2017 Sep 25;114(41):10972–10977. doi: 10.1073/pnas.1707143114

Fig. S1.

Fig. S1.

Localization of GAL4-dependent expression in brain. (A) UAS responder expressing nuclear-targeted lacZ (UAS-lacZ) was expressed under the control of the 3.0cry-Gal4 (3.0cry-G4) and MB-GS drivers. Immunostaining with LacZ antibody was performed on dissected adult fly brains and imaged by confocal microscopy. A projected Z-series image of the right brain hemisphere, where lateral is right and dorsal is top. Pacemaker neuron groups are as defined in the text. The 3.0cry-G4 drives expression in some pacemaker neurons plus new 1, new 2, and DOL cells, and MB-G4 drives expression in mushroom body neurons (MB). (A–C) A 136-μm projected Z-series image of the right brain hemisphere from a 3.0cry-G4/UAS-lacZ fly. (A) Image shown in B that includes the transmitted light view to show the outline of the brain hemisphere. (C) Magnified 58-μm projected Z-series image of the DOL region from B. (D–F) A 120-μm projected Z-series image of the right brain hemisphere from an MB-GS/UAS-lacZ induced with RU486. (D) Image shown in E that includes the transmitted light view to show the outline of the brain hemisphere. (F) Magnified 2-μm image of the MB region from E. (G–I) A 128-μm projected Z-series image of the right brain hemisphere from MB-GS/UAS-lacZ without RU486 induction. (G) Image shown in H that includes the transmitted light view to show the outline of the brain hemisphere. (I) Magnified 2-μm image of the MB region from H. (Scale bars, 10 μm.) The magnification of panels A, B, D, E, G, and H and panels C, F, and I are the same. All images are representative of six or more brains.