Table S4.
Rates of INT2 formation and deacylated tRNA release from tRNA/tRNA-labeled PRE complexes (tRNA-tRNA FRET)
| Codon | kINT2, s−1 | kINT2, release, s−1 | krelease, s−1 | kPOST, s−1 |
| UUC (COG-Phe) | 8.5 ± 0.8 | 3.5 ± 0.3 | 2.4 ± 0.1 | 2.99 ± 0.24 |
| UCU (NCOG-Phe) | 2.3 ± 0.2 | 0.8 ± 0.3 | 0.6 ± 0.1 | 0.07 ± 0.01 |
| AAA (COG-Lys) | 1.5 ± 0.1 | 1.1 ± 0.3 | 0.6 ± 0.1 | 0.62 ± 0.01 |
| AAU (NCOG-Lys) | 3.6 ± 0.3 | 0.6 ± 0.2 | 0.5 ± 0.1 | 0.11 ± 0.01 |
The rate of INT2 formation (kINT2), the rate of deacylated tRNA release after INT2 formation (kINT2, release), and the rate of deacylated tRNA release from the E site from the moment of EF-G(GTP) injection (krelease), derived from tRNA/tRNA-labeled PRE complexes bearing peptidyl-tRNAPhe or peptidyl-tRNALys in the A site as indicated (Fig. 3), are shown. Rates of translocation (kPOST) derived from analogous measurements of S13/tRNA-labeled PRE complexes (Fig. 2A) are also shown. Rates were calculated as described in Materials and Methods. COG, cognate; NCOG, near-cognate.