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Translocation rates for S13/L5-labeled PRE complexes
| Codon/Rate | kPOST, s−1 |
| AAA (COG-Lys) | 0.52 ± 0.01 |
| AAU (NCOG-Lys) | 0.10 ± 0.02 |
| AUA (NCOG-Lys) | 0.06 ± 0.01 |
| UAA (NCOG-Lys) | 0.09 ± 0.01 |
| UUC (COG-Phe) | 2.36 ± 0.43 |
| UCU (NCOG-Phe) | 0.09 ± 0.02 |
Results are similar to those obtained for S13/tRNA-labeled PRE complexes (Table 1). Translocation rates were calculated as described in Materials and Methods. COG, cognate; NCOG, near-cognate.
