Fig. S4.

Representative images comparing implanted, mock-infected and implanted, MRSA-infected bladders at 7 and 14 d. Bladders are stained for uroplakin III (red), a protein expressed on the surface of umbrella cells, cell nuclei (blue), Fg (green), and MRSA (pink). Implantation induces inflammation resulting in the exfoliation of umbrella cells (decrease in red staining), expansion of the underlying transitional cells (smaller cell nuclei, with more diffuse staining), and accumulation of Fg along the bladder cell surface (green staining). Infection with MRSA 1369 exacerbates inflammation and Fg accumulation compared with implantation alone. (Scale bars, 20 μm.)