Table S1.

Summary of equilibrium binding affinities between Get4/5 variants and Get3 variants in different substrate and nucleotide states

Get4/5 construct	Get3 complex	Nucleotide	Kd, μM
Wild type	Wild type	ATP	0.016 ± 0.002
	D57N		0.017 ± 0.003
	E251A		0.032 ± 0.008
	Wild type•Sbh1	None added	1.5 ± 0.2
	D57N•Sbh1		0.072 ± 0.006
	E251A•Sbh1		0.062 ± 0.03
	Wild type•Sbh1	AMPPNP	0.017 ± 0.004
	D57N•Sbh1		0.060 ± 0.001
	E251A•Sbh1		0.035 ± 0.007
D74K	Wild type	ATP	0.075 ± 0.002
	D57N		0.12 ± 0.04
	E251A		0.14 ± 0.09
	Wild type•Sbh1	None added	2.2 ± 0.3
	D57N•Sbh1		0.78 ± 0.08
	E251A•Sbh1		1.8 ± 0.8
	Wild type•Sbh1	AMPPNP	0.35 ± 0.01
	D57N•Sbh1		0.42 ± 0.2
	E251A•Sbh1		0.70 ± 0.4

All values are reported as mean ± SD (n ≥ 2).