Fig. 1.

Preparation of hu-mice for generating InsB:9–23-specific T cells and for induction of diabetes. (A) Schematic showing preparation of both donor hu-mice with HLA-DQ8⁺ human immune reconstitution, from which splenic human CD4⁺-naive T cells are prepared, transduced with LV-insTCR, expanded, and injected into recipient hu-mice for diabetes induction, and recipient HLA-DQ8–Tg hu-mice reconstituted with human immune cells autologous to the donor hu-mice, which are used as the recipients of LV-insTCR–transduced CD4⁺ T cells. (B). Levels (mean ± SEM) of human hematologic and immune cell chimerism in PBMCs of hu-mice from a representative experiment.