Fig. S7.

(A) Western blot analysis of PKMζ protein expression in acute hippocampal slices using anti-PKCZ antibody detected a major band at ∼55 kDa (for PKMζ) in the three lanes loaded with control (untreated), treated with antisense oligodeoxynucleotides plus SP, and scrambled plus SP (Upper). (Lower) Equal loading of samples in all three lanes using anti-tubulin antibody (50 kDa). (B) A significant decrease in the protein expression level was observed in the slices treated with PKMζ antisense oligodeoxynucleotides compared with control slices. In contrast, the protein expression was higher (equivalent to untreated control slices) when they were treated with the corresponding scrambled version of the oligodeoxynucleotides. Significant differences between groups (control vs. antisense and antisense vs. scrambled) are indicated by **P < 0.01 (slices from three biological replicates).