Fig. 2.

Cloning the tru1 gene. (A) Map-based cloning of tru1. The name of markers used for screening the recombinants are simplified as M1 to M8. Numbers of recombinants are indicated by fractions. Black boxes represent exons, and positions of transposon and ems-induced mutations are below. (B) Phylogenetic tree of BTB/POZ domain-containing homologs from selected dicots and monocots. Genes shaded in pink indicate the BOP clade, while red branches represent the monocot BOPs, including barley CUL4, maize tru1 (GRMZM2G039867), and the tru1 duplicated gene tru1-like1 (trl1) (GRMZM2G060723). Bar indicates the substitution rate per site.