Figure 2. Alginate scaffold architecture affects tissue morphology and cell behavior.

Depending on the freezing regime, the scaffolds can be prepared with isotropic (A) or anisotropic (B) pore structure. The pore dependent cell organization in the scaffold: cells cultivated in anisotropic (C, magnified in D), and spheroids grown in isotropic alginate scaffolds (E). Reprinted with permission from [32].