Figure 1.

Oxidative stress induction in dengue 2 virus- (DENV2-) infected C6/36 cells. Based on flow cytometry, the cytosolic free calcium concentration ([Ca²⁺]cyt) in DENV2-infected-C6/36 cells had slightly changed at 24 h postinfection (hpi), while it had obviously risen at 48 hpi. This reflects that endoplasmic reticular (ER) stress was activated by DENV2, even though most infected cells remained undamaged throughout the period of observation. Quantitatively, the difference in concentrations, as measured by the fluorescence intensity, between infected and uninfected cells remained low at 24 hpi but had significantly increased by 48 hpi (p < 0.05; Student's t-test). Green line: uninfected C6/36 cells; red line: DENV2-infected C6/36 cells.