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. Author manuscript; available in PMC: 2017 Oct 16.
Published in final edited form as: J Proteome Res. 2016 Nov 17;15(12):4476–4489. doi: 10.1021/acs.jproteome.6b00613

Figure 6. FAK signaling mediates invasion in BRAFi resistant cell lines.

Figure 6

(A) Western blot analysis confirms efficacy in inhibiting FAK phosphorylation with increasing doses of the FAK inhibitor, PF573228, in 1205LuR cells (2 hours after treatment). (B) Wound healing assay shows increasing concentrations of the FAK inhibitor (24 hour treatment after wound scratch) can block the increased migratory capacity of 1205LuR cells. Values represent the percentage of scratch surface area (not covered by cells). (C) Immunofluorescent viability (Calcein-AM) staining of 1205Lu and 1205LuR in 3D spheroid invasion assay. Increasing doses of FAKi block the increased invasive capacity of 1205LuR cells. (D) Matrigel invasion assay shows FAKi blocks the increased invasive capacity of 1205LuR (48 hour treatment). Cells were plated on top of Matrigel and allowed to invade towards an increased FBS gradient with and without FAKi treatment. Cells were then fixed and stained for actin cytoskeleton using Phalloidin-AlexaFluor 647. Values represent average distance of invasion in microns.