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. Author manuscript; available in PMC: 2017 Oct 16.
Published in final edited form as: J Immunol. 2016 May 16;196(12):4935–4946. doi: 10.4049/jimmunol.1402193

FIGURE 3.

FIGURE 3

Frequency and number of self-reactive 564Igi B cells in GCs of Rasgrp1-deficient mice are IL-17R dependent. (A) Representative FACS plots from spleens of 12- to 16-wk-old sufficient (far left panel) and Rasgrp1-deficient (near left panel) mice with and without IL-17R (near right and far right panels, respectively). Self-reactive 564Igi B cells were identified with an anti-idiotypic Ab, and gates were set by comparing to minus one controls (i.e., no anti-idiotypic Ab added, data not shown). Summary of the frequencies (B) and numbers (C) of 564Igi B cells from spleens of individual Rasgpr1+/− (●), Rasgrp1−/− (○), IL17R−/− (■), and Rasgrp1−/− IL17R−/− (□) mice. (D) Representative FACS plots to identify 564Igi-derived germinal center B cells in the indicated strains of mice. Frequency (E) and number (F) of 564Igi B cells localizing to GCs (one-way ANOVA with Tukey’s multiple comparison test, *p ≤ 0.03, **p ≤ 0.003, ***p ≤ 0.0003).