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. 2017 Oct 16;12(10):e0186321. doi: 10.1371/journal.pone.0186321

Fig 1. Cloning-sequencing of individual clones of the AMOT promoter CpG island.

Fig 1

Differences in the methylation of AMOT promoter CpG island in newborns are associated with prematurity. (A) Structure of the AMOT CpG proximal promoter island region. Structure of the proximal promoter region of the AMOT gene showing the location of the CpG island: the methylation CpG sites analysed were numbered 1–31. (B) Bisulfite genomic sequencing of individual clones of the AMOT promoter CpG island. Each column represents the methylation status at each CpG dinucleotide in individual clones. Each line represents an individual clone in which the methylation status is determined at each of the 31 CpG dinucleotides. Twenty nine clones from cord blood ECFC of 2 term newborns and forty clones of 3 preterm newborns were examined, respectively. Black and white dots indicate methylated and unmethylated CpGs, respectively. The positions of CpG dinucleotides further analysed by pyrosequencing are indicated by the two lines below dot plots. (C) Sequence of the AMOT promoter CpG island. Pyrosequencing primers are underlined. The nine CpG dinucleotides analysed by pyrosequencing are in bold.