Table 2. Results obtained for different sample types including: Dreissena polymorpha tissues, Dreissena bugensis tissues, D. polymorpha veligers, 1000X concentrated water, and un-concentrated water.
Information for each sample includes the location, the month and year of sample collection, sample processing, primers used, estimated target per reaction, and measured TTP.
| Sample Type | Location | Month, Year Collected | Sample Processing | Primers | Target/Reaction | Av. TTP ± SD |
|---|---|---|---|---|---|---|
| a. Direct amplification of Dreissena tissues | ||||||
| Tissue (Dreissena sp.) | Muskegon Lake (Muskegon Co., MI) | July, 2015 | Heat Treatment* | Dreissena sp. 18S rRNA | 11.12 μg | 19.67 ± 0.71 |
| 1.12 μg | 20.78 ± 0.44 | |||||
| 0.112 μg | 20.11 ± 1.05 | |||||
| 0.0112 μg | 22.56 ± 2.55 | |||||
| 0.00112 μg | 28.33 ± 7.70 | |||||
| 0.000112 μg | 35.83 ± 10.13 | |||||
| Tissue (Dreissena polymorpha) | Muskegon Lake (Muskegon Co., MI) | July, 2015 | Heat Treatment* | Dreissena polymorpha CO1 | 1111.2 μg | 22.00 ± 0.00 |
| 111.12 μg | 22.67 ± 2.08 | |||||
| 11.12 μg | 24.22 ± 2.64 | |||||
| 1.12 μg | 26.00 ± 2.55 | |||||
| 0.112 μg | 31.00 ± 4.32 | |||||
| Tissue (Dreissena bugensis) | Muskegon Lake (Muskegon Co., MI) | July, 2015 | Heat Treatment* | Dreissena bugensis CO1 | 11.12 μg | 27.13 ± 5.41 |
| 1.12 μg | 31.13 ± 5.14 | |||||
| 0.112 μg | 40.20 ± 9.78 | |||||
| 0.0112 μg | 41.00 ± 3.46 | |||||
| b. Direct amplification of Dreissena polymorpha veligers | ||||||
| Veligers (Dreissena polymorpha) | Klinger Lake (St. Joseph Co., MI) | June, 2014 | Heat Treatment* | Dreissena polymorpha CO1 | 0.09 veligers | 33.67 ± 1.15 |
| 0.009 veligers | 32.33 ± 5.69 | |||||
| 0.0009 veligers | 50.00a ± N/A | |||||
| None | 0.09 veligers | 39.67 ± 1.53 | ||||
| 0.009 veligers | 43.00 a ± N/A | |||||
| 0.0009 veligers | ND** | |||||
| c. Effect of sample collection date on results | ||||||
| Lake water concentrate (1000X) | Klinger Lake (St. Joseph Co., MI) | Oct., 2013 | Heat Treatment* | Dreissena polymorpha CO1 | N/A | ND** |
| May, 2014 | ND** | |||||
| June, 2014 | 28.67 ± 6.35 | |||||
| Lake water concentrate (1000X) | Au Train Lake (Alger Co., MI) | Nov., 2013 | Heat Treatment* | Dreissena polymorpha CO1 | N/A | ND** |
| July, 2014 | 29.67 ± 1.53 | |||||
| Aug., 2014 | ND** | |||||
| Lake water concentrate (1000X) | Antoine Lake (Dickinson Co., MI) | Nov., 2013 | Heat Treatment* | Dreissena polymorpha CO1 | N/A | ND** |
| Nov., 2014 | 22.67 ± 1.15 | |||||
| Lake water concentrate (1000X) | Higgins Lake (Roscommon Co., MI) | Oct., 2013 | Heat Treatment* | Dreissena polymorpha CO1 | N/A | ND** |
| July, 2014 | 32 ± 0.00 | |||||
| Lake water concentrate (1000X) | Gun Lake (Barry Co., MI) | Sept., 2013 | Heat Treatment* | Dreissena polymorpha CO1 | N/A | ND** |
| Oct., 2013 | ND** | |||||
| May, 2014 | 42.0 ± 5.20 | |||||
| Aug., 2014 | ND** | |||||
| June, 2015 | 32.0 ± 0.00 | |||||
aOnly 2 of 3 replicates amplified.
*Heat Treatment = 95°C for 3 min.
**ND = Not Detected