Figure 6.

Quantitative ChIP analysis of Hes1 binding to the promoter sequences of DUSP1 and PTEN. (A) Schematic diagram of the DUSP1 promoter region cantaining potential binding sites of Hes1. Immunoprecipitated DNA was amplified by PCR using primers specific for regions A and B. The arrow indicates the transcription initiation site (TIS). ATG, the translation start codon. (B) Schematic diagram of the PTEN promoter region cantaining potential binding sites of Hes1. (C) HepG2.2.15 cells were subjected to ChIP assay with mixed Hes1 antibodies or IgG antibody to identify Hes1 binding sites on the DUSP1 promoter. Two different DNA regions from the DUSP1 promoter were analyzed by qPCR. Cells were treated with DAPT or vehicle. (D) ChIP assay was performed using Hes1 antibodies or IgG to identify Hes1 binding sites on the PTEN promoter in HepG2.2.15 cells. ^**P<0.01, ^***P<0.001.