Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 19;51(5):1482–1496. doi: 10.3892/ijo.2017.4128

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Espinoza-Sánchez et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

The inducible-invasive phenotype is TGF-β independent. (A) The concentration expressed in pgs/ml of TGF-β was measured in the CM from all BrC cell lines and data were plotted. The inducible-invasive phenotype of MCF-7 and T47D cells was activated with HA-BrC CMs in the presence of 2 µg/ml of neutralizing anti-TGF-β. After 72 h of culture, (B) EMT markers were analyzed by IF and IODs of E-cadherin expression were quantified and plotted. (C) Invasion assays were performed. Left panels show representative images of invading cells and right plots show the number of invading cells. (D) Analysis of EMT markers and invasion assays of NA-BrC cell lines treated with increasing concentrations of TGF-β. Representative images are shown. Data represents the mean ± SEM from 3 independent experiments; ^***P<0.001; ns, non-significant. Scale bars indicate 100 µm, magnification of ×400 for IF and ×100 for invasion assays.

The inducible-invasive phenotype is TGF-β independent. (A) The concentration expressed in pgs/ml of TGF-β was measured in the CM from all BrC cell lines and data were plotted. The inducible-invasive phenotype of MCF-7 and T47D cells was activated with HA-BrC CMs in the presence of 2 µg/ml of neutralizing anti-TGF-β. After 72 h of culture, (B) EMT markers were analyzed by IF and IODs of E-cadherin expression were quantified and plotted. (C) Invasion assays were performed. Left panels show representative images of invading cells and right plots show the number of invading cells. (D) Analysis of EMT markers and invasion assays of NA-BrC cell lines treated with increasing concentrations of TGF-β. Representative images are shown. Data represents the mean ± SEM from 3 independent experiments; ^***P<0.001; ns, non-significant. Scale bars indicate 100 µm, magnification of ×400 for IF and ×100 for invasion assays.