|
S. cerevisiae strains |
| DKY6281 |
MATα pho8∆::TRP1 leu2-3,112 ura3-52 his3-200 trp1-901 lys2-801 suc2-9
|
|
| BJ3505 |
MATα pep4∆::HIS3 prb1-∆1.6R ura3-52 his3-200 trp1-∆101 lys2-801 can1 gal2
|
(Jones, 2002) |
| BY4741 |
MATa his3∆one leu2∆0 met15∆0 ura3∆0
|
ATCC |
|
BY4741 vam7∆::KAN
|
Invitrogen |
| AMY1018 |
BY4741 VAM7::NAT (Qc-wt knock-in; control wt strain) |
This study |
| AMY1022 |
BY4741 vam7(1-289)::NAT (Qc-1∆ knock-in) |
This study |
| AMY1021 |
BY4741 vam7(1-295)::NAT (Qc-3∆ knock-in) |
This study |
| AMY1020 |
BY4741 vam7(1-302)::NAT (Qc-5∆ knock-in) |
This study |
| AMY1019 |
BY4741 vam7(1-309)::NAT (Qc-7∆ knock-in) |
This study |
| DNY588 |
BY4741 VAM7::NAT yck3∆::KAN
|
This study |
| DNY591 |
BY4741 vam7(1-289)::NatR yck3∆::KAN
|
This study |
| DNY589 |
BY4741 vam7(1-295)::NatR yck3∆::KAN
|
This study |
| DNY590 |
BY4741 vam7(1-302)::NatR yck3∆::KAN
|
This study |
| GOY23 |
MATα pep4∆::LEU2 prb1∆::LEU2 leu2-3,112 ura3-52 his3-200 trp1-901 lys2-801 suc2-9
|
(Odorizzi et al., 1998) |
| AMY87 |
BJ3505 vam3∆
|
Merz Lab collection |
| RSY269 |
sec17-1 |
(Novick et al., 1981) |
| Yeast plasmids |
| pDN516 |
ApR
2µ URA3
|
(Nickerson et al., 2012) |
| pDN313 |
pDN516::SEC18
|
(Lobingier et al., 2014) |
| pDN314 |
pDN516::SEC17
|
(Lobingier et al., 2014) |
| pDN315 |
pDN516::SEC17 SEC18
|
(Lobingier et al., 2014) |
| pDN365 |
pDN516::sec17-(F21S, M22S — ‘FSMS’)
|
This study |
| pDN366 |
pDN516::sec17-(L291A, L292A —‘LALA’)
|
This study |
| pRS416 |
ApR
CEN URA3
|
(Sikorski and Hieter, 1989) |
| pRS426 |
ApR
2µ URA3
|
(Sikorski and Hieter, 1989) |
| pMS120 |
VAM7 (pRS426; Qc-wt overproduction) |
This study |
| pMS121 |
vam7(1-289) (pRS426; Qc-1∆ overproduction) |
This study |
| pMS122 |
vam7(1-295) (pRS426; Qc-3∆ overproduction) |
This study |
| pMS123 |
vam7(1-302) (pRS426; Qc-5∆ overproduction) |
This study |
| pMS124 |
vam7(1-309) (pRS426; Qc-7∆ overproduction) |
This study |
| pMS125 |
VAM7 (pAG25) |
This study |
| AMP1492 |
pRS416::SEC17
|
This study |
| AMP1774 |
pRS416::sec17-K159E |
|
| AMP1774 |
pRS416::sec17-K163E |
This study |
| AMP1774 |
pRS416::sec17-K159E,K163E (‘KEKE’) |
This study |
|
E. coli plasmids |
| AMP356 |
pET41::His6-TEV-VAM7-intein-CBD
|
(Schwartz and Merz, 2009) |
| AMP359 |
pET41::His6-TEV-VAM7(1–289, 1∆)-intein-CBD
|
(Schwartz and Merz, 2009) |
| AMP1809 |
pET41::His6-TEV-VAM7(1–291, 2∆)-intein-CBD
|
This study |
| AMP358 |
pET41::His6-TEV-VAM7(1–295, 3∆)-intein-CBD
|
(Schwartz and Merz, 2009) |
| AMP1810 |
pET41::His6-TEV-VAM7(1–299, 4∆)-intein-CBD
|
This study |
| AMP357 |
pET41::His6-TEV-VAM7(1–302, 5∆)-intein-CBD
|
(Schwartz and Merz, 2009) |
| AMP1806 |
pET41::His6-TEV-VAM7(1–302, AR-5∆)-intein-CBD
|
This study |
| AMP1807 |
pET41::His6-TEV-VAM7(1–302, RA-5∆)-intein-CBD
|
This study |
| AMP1808 |
pET41::His6-TEV-VAM7(1–302, AA-5∆)-intein-CBD
|
This study |
| AMP360 |
pET41::His6-TEV-VAM7(1–309, 7∆)-intein-CBD
|
(Schwartz and Merz, 2009) |
| AMP1547 |
pTYB12::intein-CBD-SEC17
|
(Schwartz and Merz, 2009) |
| AMP1547 |
pTYB12::intein-CBD-SEC17 (F21R, M22S, L24S, F25R) |
This study |
| AMP1548 |
pTYB12::intein-CBD-SEC17 (F21R, F25R) |
This study |
| AMP1549 |
pTYB12::intein-CBD-SEC17 (∆21–25) |
This study |
| AMP1550 |
pTYB12::intein-CBD-SEC17 (F21S, M22S — ‘FSMS’) |
This study |
| AMP1551 |
pTYB12::intein-CBD-SEC17 (∆1–26) |
This study |
| AMP1777 |
pTYB12::intein-CBD-SEC17 (K159E) |
This study |
| AMP1778 |
pTYB12::intein-CBD-SEC17 (K163E) |
This study |
| AMP1779 |
pTYB12::intein-CBD-SEC17 (K159E, K163E — ‘KEKE’) |
This study |
