Table 2.
Diet∗ | Sex† | HSI | KSI | SSI | WBC 103 | Monocytes% | Neutrophils% | Lymphocytes% | ALT (UL−1) |
---|---|---|---|---|---|---|---|---|---|
1 | F | 0.88 ± 0.03a | 0.15 ± 0.002a | 0.05 ± 0.001a | 19.30 ± 0.28a | 3.56 ± 0.003a | 0.37 ± 0.001a | 10.82 ± 0.2a | 41.96 ± 0.08a |
2 | 1.42 ± 0.02b | 0.24 ± 0.001b | 0.07 ± 0.001a | 20.29 ± 0.24a | 3.05 ± 0.005b | 0.26 ± 0.008b | 16.50 ± 0.01b | 42.56 ± 0.06a | |
3 | 1.94 ± 0.05c | 0.33 ± 0.003c | 0.11 ± 0.002b | 28.26 ± 0.85b | 0.52 ± 0.004c | 2.01 ± 0.01c | 29.21 ± 0.18c | 46.82 ± 0.05b | |
4 | 2.97 ± 0.04d | 0.42 ± 0.005d | 0.34 ± 0.12c | 37.97 ± 0.08c | 0.37 ± 0.003d | 3.97 ± 0.005d | 30.75 ± 0.03cd | 50.50 ± 0.08c | |
| |||||||||
1 | M | 0.84 ± 0.03a | 0.15 ± 0.002a | 0.05 ± 0.001a | 19.45 ± 0.29a | 3.58 ± 0.03a | 0.38 ± 0.001a | 10.95 ± 0.02a | 41.99 ± 0.06a |
2 | 1.40 ± 0.02b | 0.24 ± 0.001b | 0.07 ± 0.001a | 20.47 ± 0.25a | 3.06 ± 0.05b | 0.28 ± 0.006b | 16.70 ± 0.01b | 42.60 ± 0.08a | |
3 | 1.89 ± 0.05c | 0.34 ± 0.003c | 0.11 ± 0.02b | 28.45 ± 0.85b | 0.54 ± 0.03c | 2.02 ± 0.01c | 29.36 ± 0.10c | 46.84 ± 0.08b | |
4 | 2.94 ± 0.04d | 0.42 ± 0.004d | 0.33 ± 0.13c | 38.16 ± 0.08c | 0.38 ± 0.03d | 3.98 ± 0.004d | 30.83 ± 0.06d | 50.69 ± 0.05c | |
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ANOVA | |||||||||
Aflatoxin∗ sex | 0.97 | 1 | 1 | 1.00 | 0.99 | 0.99 | 0.98 | 0.68 | |
Sex | 0.10 | 0.62 | 0.98 | 0.62 | 0.19 | 0.33 | 0.18 | 0.25 | |
Aflatoxin (dose) | <0.05 | <0.05 | <0.05 | <0.05 | <0.05 | <0.05 | <0.05 | <0.05 |
†M: male O. niloticus; F: female O. niloticus with n = 10 (10 individual fish per treatment (10 males and 10 females) were measured); ∗diet 1: 0 μg kg−1 (control); diet 2: 20 μg kg−1; diet 3: 200 μg kg−1; diet 4: 2000 μg kg−1. ∗Means followed by similar letters do not differ significantly (P < 0.05).