Fig 4. Normal cell death but decreased cell proliferation of in MiR125a-/- neutrophils.
(A) Apoptosis of bone marrow neutrophils in MiR125a+/+ and MiR125a-/- mice. 2x106 bone marrow cells were cultured in 10% FBS 1640 medium for 48 hours. Flow cytometry analysis of Ly6G+ cell apoptosis by using Annexin V and PI staining. The bar graph shows the percentage of Annexin V + neutrophils (mean±s.d., n = 3). (B-C) Flow cytometry analysis of neutrophils incorporating BrdU in bone marrow (B) and spleen (C) after in vivo pulsing BrdU for 72 hours. Neutrophils were previously gated with CD11b-Percp cy5.5 and Ly6G-APC. Bar graphs indicate the mean percentage of BrdU-incorporating neutrophils (mean±s.d., n = 3). ns, none significant difference, *P<0.05 (Student’s t-test).