Fig 5. Decreased proliferation of immature neutrophils in MiR125a^-/- mice.

(A) Flow cytometry analysis of three subpopulations in CD11b⁺Gr-1⁺ neutrophils in bone marrow. Mature neutrophils (mNeu) indicate CD11b^hi Gr-1^hi cells. Immature neutrophils (imNeu) indicate CD11b^lowGr-1^hi cells and promyelocytes/myelocytes (pro/mye) indicate CD11b^intGr-1^int cells. The bar graph shows the average numbers of these subpopulations in MiR125a^+/+ and MiR125a^-/- mice. Values were represented as mean±s.d., n = 5 mice of each genotype. (B) Flow cytometry analysis of three populations of CD11b⁺Gr-1⁺ neutrophils incorporating BrdU in bone marrow after in vivo pulsing BrdU for 72 hours. The bar graph indicates the average percentage of intensities of BrdU-incorporating cells (mean±s.d., n = 5). Ns, none significant difference, *P<0.05, ***P<0.001 (Student’s t-test).