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Kissing-loop complementarity is necessary for 3′X and 5BSL3.2 to form a RNA:RNA complex. The mixtures of 5BSL3.2 with 3′X and its lr-AID mutants were examined by native polyacrylamide gel electrophoresis. Both 3′X3SL-lrAID and 3′X formed a complex with 5BSL3.2, whereas no interactions between 3′X2SL-lrAID and 5BSL3.2 were detected.
