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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Clin Exp Pharmacol Physiol. 2017 Aug 30;44(11):1134–1144. doi: 10.1111/1440-1681.12813

Figure 2. Prorenin induces ROS formation at nanomolar range and ROS scavenger p-coumaric acid prevented this effect.

Figure 2

M-1 cells were incubated with prorenin and renin (10-12, 10-10 , 10-8 , 10-6 M). ROS production was measured after 10 min using DCFHDA probe and levels of DCF formed (nM/μg total protein). ROS formation was augmented by prorenin at 10-8 M. Renin increases ROS formation at non-physiological dose (10-5 M). P-coumaric acid (PCA, 10-7 M) added 10 min before prorenin treatment prevented the induction of ROS. As a positive control 200 mM H2O2 was used. *P<0.05 versus control, ***P<0.001 versus control, n=12.