Skip to main content
. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Apoptosis. 2017 Nov;22(11):1380–1393. doi: 10.1007/s10495-017-1409-8

Figure 3. Prenylation is not requisite for nuclear localization of Rac 1.

Figure 3

Panel A: Rat pancreatic islets were isolated and pre-incubated with GGTI-2147 for overnight prior to treatment with LG or HG for 24 hours. Nuclear accumulation of Rac1 was determined by Western blotting.

Panel B: Pooled data from three independent experiments are shown in the graph. ** p<0.005 vs WT Rac1.

Panel C: INS-1 832/13 cells were transfected with GFP wild type [WT] or unprenylatable and constitutively active GFP Rac-1 [Rac1 SAAX], unprenylatable Rac1 [Rac1 C189A] and relative abundance of Rac1 in the non-nuclear and nuclear fractions was assessed by Western blotting followed by densitometry. Lamin B was used as marker for the nuclear fraction.

Panel D: INS-1 832/13 cells were transfected with GFP wild type [WT] or unprenylatable and constitutively active GFP Rac-1 [Rac1 SAAX], unprenylatable Rac1 [Rac1 C189A] and localization of Rac1 was analyzed using fluorescent microscope. DAPI was used to counterstain the nucleus.