Skip to main content
. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Apoptosis. 2017 Nov;22(11):1380–1393. doi: 10.1007/s10495-017-1409-8

Figure 6. Metformin inhibits HG- induced p38MAPK and p53 activation in INS-1 832/13 cells.

Figure 6

Panel A: INS-1 832/13 cells were incubated with LG [2.5mM] and HG [20mM] in the presence and absence of metformin [0–30 μM] for 24 h. Relative abundance of phopho-p38MAPK and total-p38MAPK was determined by Western blotting.

Panel B: Quantification of the phopho-p38 bands was done by densitometry and the ratios were calculated over total-p38 in the presence of metformin [n=5]. *p < 0.05 vs 2.5mM glucose alone, **p < 0.05 vs 20mM glucose alone; NS: not significant.

Panel C: INS-1 832/13 cells were treated with LG [2.5mM] and HG [20mM] in the absence or presence of metformin [0–30 μM] for 24 h. Cell lysates were separated and analyzed for phosphorylated and total p53 by Western blotting.

Panel D: Band intensities for phospho-p53 were measured using densitometry and the ratios were calculated over total-p53 in the presence of metformin. *p < 0.05 vs 2.5mM glucose alone, **p < 0.05 vs 20mM glucose alone [n=3].