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. 2017 Oct 16;7:13260. doi: 10.1038/s41598-017-13550-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OM- and periplasmic-MOSP form SDS-stable trimers and distinct multimeric complexes. (A) Protocol outlining the use of NP-40 to separate OM and periplasmic conformers. (B) Immunoblot of the NP-40 soluble (supernatant; NP-40 Sup) and insoluble (pellet; NP40 Pel) fractions obtained by ultracentrifugation which were then separately subjected to TX-114 phase partitioning (TX-114 pp). (C) Immunoblot of BN-PAGE gels of NP-40 supernatant and TX-114 aqueous phase using anti-MOSP^Fl antiserum. Also shown are SDS-PAGE gels of the separated conformers without (−) and with (+) boiling followed by immunoblot analysis with anti-MOSP^Fl. Molecular mass standards (kDa) are indicated on the left (SDS-PAGE) or right (BN-PAGE) of each gel.