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. 2017 Oct 16;7:13260. doi: 10.1038/s41598-017-13550-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The dentilisin complex associates only with OM-MOSP. (A) SDS-PAGE and immunoblot analysis of TX-114 phase partitioned T. denticola cells using antisera directed against PrtP, PrcA1, PrcA2, and PrcB. Lanes: whole cells (WC), TX-114 insoluble material (Ins), aqueous (Aq) and detergent (Det) phase. (B) SDS-PAGE gels of the NP-40 supernatant without (−) or with (+) boiling followed by immunoblot analysis using the same antisera as in Panel A. (C) Eluates from Co-IP of OM and periplasmic conformers were immunoblotted with antisera against the four dentilisin components. (D) SDS-PAGE and immunoblot analysis of eluate when 2% DDM was added to the NP-40 supernatant prior to Co-IP. Molecular mass standards (kDa) are indicated on the left of each gel.