Figure 5.

The biological effect of HCG18 on NP could be reversed by regulating the expression of TRAF6. NP cells were first infected with TRAF6 or TRAF6 shRNA virus (MOI = 50) for 48 h. Then, the cells were transfected with the HCG18 expression vector, shRNA and control for 48 h. (A) TRAF6 protein level was measured by western blot in NP tissues. Full-length blots are presented in Supplementary Figures S3. (B) Cell viability was measured with the CCK-8 assay. (C) Quantification of Ki67 labelled nucleus in NP cells. (D–E) Cell cycle and apoptosis rate were measured by flow cytometry. (F) Quantification of the number of invasive macrophages. (G–H) ALP staining and Alizarin red S staining were quantified using the absorbance value 7 days after incubation. *P < 0.05.