Fig. 6.

MIIP-S303 phosphorylation is required for tumor metastasis and is related to poor prognosis in human colorectal cancer. a SW620 cells with reconstituted expression of WT rMIIP, rMIIP S303A and rMIIP S303A/HDAC6 shRNA were intraspleenically injected into the athymic nude mice. Representative tumor xenografts were shown (left panel). The number of visible metastatic lesions in the liver was measured. Data represent the means±s.e.m. (n = 8, right panel), * represents P < 0.05 (Student’s t-test). b Lysates obtained from the pool of tumor tissues (n = 8) developed from SW620 cells (as indicated in Fig. 6a) were subjected to immunoblotting analyses using the indicated antibodies. c Immunohistochemical staining with anti-MIIP pS303 was performed on 182 human colorectal cancer specimens. Representative photos of non-metastasis and metastasis tumor versus the adjacent normal tissues were shown (magnification: ×100 and ×400). Scale bars: 100 μm. d MIIP pS303 levels in tumor and the adjacent normal tissues of colorectal cancer. e MIIP pS303 levels in tumor metastasis (M1) or non-metastasis subgroups (M0). The Chi-square test indicating the tight correlation between MIIP pS303 levels and tumor metastasis. f The survival times for 182 patients with low (score < 6, blue curve) versus high ((score ≥ 6, red curve) MIIP pS303 levels (low, 73 patients; high, 109 patients). The Kaplan–Meier method and log-rank tests indicating the significant association of MIIP pS303 levels (P = 0.0235) with patient survival. In a, d, * represents P < 0.05 and ** represents P < 0.01 (Student’s t-test) between indicated groups