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. 2017 Oct 16;7:13220. doi: 10.1038/s41598-017-13576-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The level of Can1-GFP does not differ in the mother cells aging cohorts. (a) Representative photograph of the control yeast cells expressing Can1-GFP. (b) Can1-GFP levels in the individual cells of different replicative age cohorts (n = 410 cells). Grey boxplots represent the random samples of yeast cells, the red box plot represents TRITC+ (age > 4 enriched) mother cells. The numbers of analyzed cells for each age class are shown at the top of the boxplot. The bin size for TRITC+ and TRITC− cells are designated separately. P value was calculated according Nemenyi-Test.