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. 2017 Oct 16;7:13220. doi: 10.1038/s41598-017-13576-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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103Q-CFP level displays mother-daughter asymmetry. (a) Representative photograph of the group of cells expressing 103Q-CFP (red) stained with calcofluor white (blue). Image was generated by pseudo colouring: CFP-channel black and white image was copied to R channel, CW-channel image — to G and B channels of the RGB file. The dotted circles mark daughter cells. (b) Quantification of the average 103Q-CFP levels for replicative age cohorts. The numbers of analyzed cells for each age class are shown at the top of the boxplot. P value was calculated by Nemenyi-Test.