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. 2017 Oct 16;7:13302. doi: 10.1038/s41598-017-13440-x

Table 1.

Significantly regulated phosphosites in siMCM2 phosphoproteome with no change in protein abundance from the global siMCM2 proteome in H1299 lung cancer cells.

Protein Proteome Log 2 ratio (siMCM2/CTL) a Phosphoproteome Log 2 ratio (siMCM2/CTL) b Phosphorylation sites on protein c Peptide sequence d Phosphorylation sites on peptide e
BOD1L1 0.09 0.73 Ser2779; 2780 _QHYLpSpSEDEPDDNPDVLDSR_ 5; 6
BOP1 −0.31 −0.59 Ser126; 127 _IGDEYAEDpSpSDEEDIR_ 9; 10
CANX 0.22 0.71 Ser564 _QKSDAEEDGGTVpSQEEEDR_ 13
CANX 0.22 −0.94 Thr562 _QKSDAEEDGGpTVSQEEEDR_ 11
CCDC132 −0.09 −0.67 Ser559 _SAYQEYDpSDSDVPEELK_ 8
CCDC86 −0.21 −1.08 Ser69 _AGLGSPERPPKTSPGpSPR_ 16
CCDC86 −0.21 −0.98 Ser110 _QQDLHLESPQRQPEYpSPESPR_ 16
CCDC86 −0.21 −0.84 Thr65 _AGLGSPERPPKpTSPGSPR_ 12
CLIP1 0 0.83 Ser200; 204 _TASESISNLpSEAGpSIK_ 10; 14
DBN1 0.33 0.9 Ser141 _LpSSPVLHR_ 2
DEK −0.23 −1.34 Ser301; 303; 306; 307 _KEpSEpSEDpSpSDDEPLIKK_ 3; 5; 8; 9
ECM29 0.05 -4.55 Ser1419 _pSCAFAMGHLVRTSRDSSTEK_ 1
HDGF −0.07 −2.7 Ser132; 133 _KGNAEGpSpSDEEGKLVIDEPAK_ 7; 8
HIST1H1B −0.04 −1.23 Ser18 _SETAPAETATPAPVEKpSPAK_ 17
HMGA1 0.05 −2.24 Ser99 _KLEKEEEEGIpSQESSEEEQ_ 11
HMGA1 0.05 −4.19 Ser102; 103 _KLEKEEEEGISQEpSpSEEEQ_ 14; 15
HNRNPUL2 −0.08 0.7 Ser161 _REEDEPEERpSGDETPGSEVPGDK_ 10
HNRNPUL2 −0.08 0.82 Thr165 _REEDEPEERSGDEpTPGSEVPGDK_ 14
MYBBP1A 0.11 −1.96 Ser1159; Thr1161 _EIPpSApTQSPISK_ 4; 6
MYH9 0.01 −0.87 Ser1943 _GAGDGpSDEEVDGKADGAEAKPAE_ 6
NCL 0.03 −0.93 Ser563 _LELQGPRGpSPNAR_ 9
NOC2L 0.04 −0.73 Ser49 _EAARpSPDKPGGSPSASR_ 5
NOC2L 0.04 −0.74 Ser56 _EAARSPDKPGGpSPSASR_ 12
NUMA1 0.07 −1.58 Ser1225; 1235 _NpSLISSLEEEVpSILNR_ 2; 12
PA2G4 −0.02 0.68 Thr386 _TAENATSGEpTLEENEAGD_ 10
PDAP1 −0.06 −0.65 Ser60; 63 _SLDpSDEpSEDEEDDYQQK_ 4; 7
PGRMC2 0.04 −0.88 Thr211 _LLKPGEEPSEYpTDEEDTK_ 12
PLEC −0.08 −0.78 Ser1435 _AQLEPVApSPAK_ 8
PPP1R12A −0.16 −0.67 Ser862 _STGVSFWTQDpSDENEQEQQpSDTEEGSNKK_ 11
PPP1R12A −0.16 −1.73 Ser445 _TGpSYGALAEITASK_ 3
PPP1R12A −0.16 −3.38 Thr443 _pTGSYGALAEITASK_ 1
RANBP2 0.11 0.72 Thr1393; Ser1400 _ELVGPPLAETVFpTPKTpSPENVQDR_ 10; 17
RBM14 −0.19 0.66 Ser582 _TRLpSPPR_ 4
RPS6 0.03 −0.71 Ser235 _RLpSSLRASTSK_ 3
RRM2 −0.42 −0.71 Ser20 _VPLAPITDPQQLQLpSPLK_ 15
RRP1B 0.00 0.61 Ser245 _VGDGDLpSAEEIPENEVSLR_ 7
RTN4 0.26 0.7 Ser184 _RGSSGpSVDETLFALPAASEPVIR_ 6
SEC. 31 A −0.1 0.6 Ser532 _DSDQVAQSDGEEpSPAAEEQLLGEHIK_ 13
SPTBN1 0.26 0.65 Ser2164 _EpSSPIPSPTSDRK_ 2
SPTBN1 0.26 0.7 Ser2165 _TSSKESpSPIPSPTSDRK_ 7
SRRM2 0.06 −1.16 Ser351 _SATRPpSPSPERSSTGPEPPAPTPLLAER_ 6
SRRM2 0.06 −0.86 Ser353 _SATRPSPpSPERSSTGPEPPAPTPLLAER_ 8
SRRM2 0.06 −1.02 Ser357 _SATRPSPSPERpSSTGPEPPAPTPLLAER_ 12
SRRM2 0.06 −1.71 Ser2692; 2694 _SLpSYpSPVER_ 3; 5
SRSF1 −0.18 −0.96 Ser201; 205 _VDGPRSPpSYGRpSR_ 8; 12
ST13 0.23 0.59 Ser79 _KVEEDLKADEPSSEEpSDLEIDK_ 16
STUB1 −0.17 −0.69 Ser19; 23 _LGAGGGpSPEKpSPSAQELK_ 7; 11
THRAP3 −0.19 0.6 Ser379 _GSFpSDTGLGDGK_ 4
TNKS1BP1 0.23 0.64 Ser1666 _NRpSAEEGELAESK_ 3
TUBA1C 0.18 −0.71 Ser48 _TIGGGDDpSFNTFFSETGAGK_ 8
UTP18 −0.33 −1.11 Ser205 _RKTpSSDDESEEDEDDLLQR_ 4
UTP18 −0.33 −0.76 Ser210 _TSSDDEpSEEDEDDLLQR_ 7

aLog2 value of the normalized siMCM2/siRNA control ratio from siMCM2 proteome in H1299 lung cancer cells.

bLog2 value of the normalized siMCM2/siRNA control ratio from siMCM2 phosphoproteome in H1299 lung cancer cells.

cPosition(s) of the phosphorylated residue within this protein.

dSequence of matched peptide including the location(s) of phosphorylated amino acid.

ePosition(s) of the phosphorylated residues within this peptide.