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. 2017 Oct 16;7:13248. doi: 10.1038/s41598-017-13251-0

Figure 3.

Figure 3

Exogenous NaHS promoted glucose uptake and increased the phosphorylation of insulin signalling pathway components in C2C12 cells. After insulin resistance was induced by PA, culture medium was completely removed and replaced with NaHS solution. NaHS solution was freshly prepared and diluted to 50 μM with DMEM. (A) Cells were incubated in NaHS solution for 30 minutes at 37 °C, followed by incubation with 10 nM insulin and 150 μg/ml 2-NBDG. NaHS treatment promoted glucose uptake in both control C2C12 cells and the IR cell model. (B) Phosphorylation levels of IRS1, PI3K and AKT were evaluated by Western blot. In the PA-treated group, phosphorylation of IRS1, PI3K and AKT were decreased. Administration of NaHS increased the phosphorylation of IRS1, PI3K and AKT. *p < 0.05, **p < 0.01, ***p < 0.001. Data are shown as the mean ± sem.