Table 1.
Information about the antibodies.
| Peptide/Protein Target | Name of Antibody | Manufacturer; Catalogue No. | Host Species, Monoclonal or Polyclonal | Dilution |
|---|---|---|---|---|
| CBS | Anti-CBS antibody | Abcam; ab135626 | Rabbit polyclonal | 1:1000 |
| MPST(D-8) | MPST antibody (D-8) | Santa Cruz Biotechnology; sc-374326 | Mouse monoclonal | 1:1000 |
| CSE | CSE monoclonal antibody | Proteintech; 60234-1-lg | Mouse monoclonal | 1:500 |
| Actin | Anti-beta-actin monoclonal antibody | Lianke; Mab1445 | Mouse monoclonal | 1:3000 |
| IRS1 (phospho Ser-312) | IRS1 (phospho Ser 312) antibody | GeneTex; GTX24865 | Rabbit polyclonal | 1:1000 |
| IRS1 | Anti-IRS1 antibody | Millipore; 04-784 | Rabbit monoclonal | 1:1000 |
| Phospho-PI3 kinase p85 (Tyr-458)/p55 (Tyr-199) antibody | Phospho-PI3 kinase p85 (Tyr458)/p55 (Tyr199) | Cell Signaling Technology; 4228 | Rabbit polyclonal | 1:1000 |
| PI3K | P85;PI3R1 monoclonal antibody | Proteintech; 60225-1-Ig | Mouse monoclonal | 1:1000 |
| AKT (phospho Ser-473) | AKT1 phospho (pS473) Rabbit monoclonal antibody | Epitomics; 2118-1 | Rabbit monoclonal | 1:1000 |
| AKT | AKT 1 + 2 + 3 antibody | GeneTex; GTX121937 | Rabbit polyclonal | 1:4000 |
| phospho-AMPK (Thr-172) | p-AMPKα1/2 Antibody | Santa Cruz Biotechnology; sc-33524 | Rabbit polyclonal | 1:600 |
| AMPKα1/α2 | AMPKα1/AMPKα2 (ab-174/ab-172) antibody | Signalway Antibody; 21191 | Rabbit polyclonal | 1:1000 |
| OXPHOS | Anti-Rt/Ms Total Oxphos Complex Kit | lifetechnologies | Mouse monoclonal | 1:1000 |