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. 2017 Oct 16;7:13289. doi: 10.1038/s41598-017-13632-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phase contrast-enhanced visualisation of the soft tissue comprising blood vessels in calcified bone. (a) Schematic representation of an intracortical canal occupied by a blood vessel. Image intensity profile plotted along a line intercepting intracortical canal and internal blood vessel for standard X-ray micro-computed tomography (µCT) (b). Magnified area on µCT reconstructed slice containing an intracortical canal (0.325 µm voxel size, 15 mm propagation distance) (c). Image intensity profile plotted along a line intercepting intracortical canal and internal blood vessel for phase contrast-enhanced µCT (d). Magnified area on phase contrast-enhanced µCT reconstructed slice containing an intracortical canal with internal blood vessel visible (0.325 µm voxel size, 25 mm propagation distance) (e).