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. 2017 Oct 16;7:13289. doi: 10.1038/s41598-017-13632-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D visualisation and extraction of intracortical blood vessel. (a) 3D rendering of murine tibia with identification of the tibiofibular junction. (b) 3D rendering of scanned tibiofibular junction region (1.3 µm voxel size) and (c) detection of intracortical canals (blue) as a negative imprint of the mineralised tissue (extracted from 1.3 µm voxel size dataset). (d) Magnified area of the 3D intracortical network (extracted from 0.325 µm voxel size dataset) and (e) detection of the soft tissue comprising blood vessels (red) within intracortical canals (blue) (extracted from 0.325 µm voxel size dataset). (f) Magnified segment of blood vessel (red) within intracortical canal (blue) (extracted from 0.325 µm voxel size dataset).