Fig. 8.

Conditional ablation of Runx1 in ILCP. a Top PLZF expression in BM Lin^-IL7Rα⁺ α4β7⁺ cells from Il7ra-Cre and Il7ra-Cre Runx1 ^fl/fl. BM samples were MACS-enriched using anti-α4β7 antibody before staining with other antibodies. Summary data of ILCP cell number and PLZF mean fluorescence intensity of ILCP cells are combined from seven independent experiments with 15 and 12 mice in each group. Bottom, Runx1 and PLZF staining in BM Lin^-IL7Rα⁺ α4β7⁺ cells from Il7ra-Cre and Il7ra-Cre Runx1 ^fl/fl. Data are representative of three mice from each group. b PLZF expression in BM Lin^-IL7Rα⁺ α4β7⁺ cells from Il7ra-Cre and Il7ra-Cre Cbfb ^fl/fl. Data are summarized from four independent experiments, with 8–11 mice in each group. Statistical analysis was performed using two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001