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. 2017 Oct 16;7:13214. doi: 10.1038/s41598-017-13575-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Isolation of the PSI complex from Chromera. (A) Solubilised membranes were loaded on a sucrose gradient and centrifuged at 75 000 x g for 18 hours; the SG1 (PSI) fraction was then collected and separated on a size-exclusion column (B). Eluted proteins and complexes were detected by a diode-array detector and a fluorescence detector set to 440/680 nm (excitation/emission wavelengths). Chlorophyll absorbance (670 nm) is shown by a green line and chlorophyll fluorescence is shown by a red dotted line; FCP, free antenna proteins. PSI complex, represented by peak 6-7.5 mL, was collected; see also Supplementary Fig. S3.