Figure 2.

SRVF induces cell cycle arrest at G₂/M phase in HT1080 cells. (A) Cells were incubated with SR, VF, or SRVF at a concentration of 250 µg/ml for 12 or 24 h, and cell cycle distribution was examined by flow cytometry after staining intracellular DNA with propidium iodide solution. Cell cycle distribution was analyzed using Kaluza software and is expressed as the relative percentage. Data are representative of three independent experiments. (B) The expression of cell cycle-related proteins was examined by Western blot analysis in cells treated with SR, VF, or SRVF at a concentration of 250 µg/ml for 12 h. Relative band intensities were calculated using ImageJ software after normalizing to tubulin expression. The full size blot is shown in Supplementary Fig. S2.