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. 2017 Oct 16;8:959. doi: 10.1038/s41467-017-00931-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Late-stage thymocyte development is defective in NCoR1-deficient mice. a, b Quantitative RT-PCR analysis of Ncor1 mRNA levels in indicated tissues (a, n = 5), and sorted CD4⁻CD8⁻ double-negative (DN) thymocytes (n = 3) and CD4⁺CD8⁺ double-positive (DP) (n = 6) (b) thymocytes from NCoR1-deficient (cKO) and wild-type (WT) mice. c, d Total cell numbers (c) and surface expression of CD4 and CD8 (d) on thymocytes from cKO (n = 5) and WT (n = 5) mice detected by flow cytometry. e Absolute cell numbers of DP, CD4⁺ or CD8⁺ single-positive (SP) thymocytes from cKO and WT mice (n = 5 for each group), as the product of total thymocytes multiplied by the percentage of cells found in that population. f Expression profiles of CD62L vs. CD69 or CD24 on cKO (n = 3) and WT (n = 3) CD4 SP thymocytes detected by flow cytometry. g Cell numbers of each subpopulation gated in (f). h Expression of CD4 and CD8 (right) from mix-bone marrow chimeras generated from CD45.1^+/− WT and CD45.2⁺ cKO (left) donors (n = 4 to 5). i Quantification of each subpopulation gated in (h). The data are representative of two independent experiments (a, b, h) or three independent experiments (c–f). Statistical significance was analyzed using the two-tailed Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant)