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. 2017 Oct 16;8:959. doi: 10.1038/s41467-017-00931-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — NCoR1 is required to prevent excessive negative selection. a Flow cytometry analysis of 5 subpopulations (left) by MHCI and CD69 expression on total NCoR1-deficient (cKO) and wild-type (WT) thymocytes (n = 3 to 5). The right panel shows CD4 and CD8 expression on gated population #3. b The proportion of population #3 (left) and CD4^dullCD8^dull subsets in population #3 (right). c Intracellular levels of Act.Casp.3 detected by flow cytometry in the CD69^hiMHCI⁻CD4^dullCD8^dull subsets from cKO and WT mice (n = 4 to 5 for each group). d Flow cytometry analysis of CD4 and CD8 expression on total thymocytes 6 weeks after transferring bone marrow cells from OT-II TCR-transgenic cKO or WT mice into irradiated rat insulin promoter-ovalbumin (RIP-OVA) transgenic mice (n = 4 to 5). e TCR Vα2 expression of total thymocytes from the mice in (d). The data are representative of three independent experiments (a, b) or two independent experiments (c, d). Statistical significance was analyzed using the two-tailed Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001)