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. 2017 Oct 16;7:13240. doi: 10.1038/s41598-017-12753-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunostaining of MCETs with histone and tryptase on in vitro interaction of RBL MCs with L. donovani. Cells cultured on a cover glass slip for overnight incubated with L. donovani (green) for 24 h at an MOI of 1:10 in RBL medium and were processed as discussed in materials and methods. Panel a represent staining with anti – histone and panel b represent staining with anti – tryptase and were visualized under confocal microscopre at 60X. Scale bar is 50 μm, n = 3.