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. 2017 Oct 16;7:13240. doi: 10.1038/s41598-017-12753-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evidence of extracellular killing of L. tropica and L. donovani by Mast Cell Extracellular Traps. 0.1 × 10⁶ MCs were seeded in 48 well cell culture plate and cultured overnight in CO₂ incubator. Panel 8a and 8b represent recovery of RBL MCs (% of total) by trypan blue on co-culture with L. tropica and L. donovani pretreated with DNase. Panel 8c and 8d represent recovery of PMCs (% of total) by MTT assay co-culture with L. tropica and L. donovani pretreated with DNase. Each point represents mean ± SEM of values obtained from three independent assays.