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. 2017 Oct 12;7:440. doi: 10.3389/fcimb.2017.00440

Figure 2.

Figure 2

Detection of Mycoplasma contamination in infected C3A cells. Lane 1: day 2 culture; lane 2: day 4 culture; lane 3: day 6 culture; lane 4: positive control; lane 5, negative control. (B) Mycoplasma detection in Tc and M(+) cell groups. Lane 1: 1d M(+) cell; lane 2: 8d M(+) cell; lane 3: 15d M(+) cell; lane 4: 22d M(+) cell; lane 5: 27d M(+) cell; lane 6: 36d M(+) cell; lane 7: 43d M(+) cell; lane 8: 1d Tc; lane 9: 8d Tc; lane 10: 15d Tc; lane 11: 22d Tc; lane 12: 29d Tc; lane 13: positive control; lane 14: negative control. (C) Monoclonal human–mice cell contamination. Lane 1: Tc+ human COX-I primers; lane 2: M(−) cell+ human COX-I primers; lane 3: ML12+human COX-I primers; lane 4: ddH20+human COX-I primers; lane 5: Tc+ mice COX-I primers; lane 6: M(−) cell+mice COX-I primers; lane 7: AML12+mice COX-I primers; lane 8: ddH20+mice COX-I primers; lane M, 1-2000-bp DNA marker (Takara).