Figure 7.

KCa3.1 is involved in ER stress in vivo. Focal cerebral ischemia was induced by pMCAO in WT and KO mice. (A–C) Western blot analysis of lysates from 10-week-old male WT and KO mice 1, 3, 6 and 24 h after pMCAO, analyzed by antibodies to GFAP and GRP78. Data represent means ± SEM of GFAP and GRP78 density, normalized to β-actin values (n = 3). *p < 0.05 compared with control, 0 h. (D) Double immunofluorescence staining of GRP78 (green) with GFAP (red) in brain sections of WT and KO pMCAO (6 h) mice. Nuclei were stained in blue with DAPI. Scale bar: 25 μm. WT, wild type; KO, knockout.