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. 2017 Oct 12;11:319. doi: 10.3389/fncel.2017.00319

Figure 8.

Figure 8

Involvement of KCa3.1 in pMCAO mouse model through ERK1/2 and JNK signaling pathways. Focal cerebral ischemia was induced by pMCAO in WT and KO mice. (A–D) Western blot analysis of lysates from 10-week-old male WT and KO mice 1, 3, 6 and 24 h after pMCAO, analyzed by antibodies to phosphorylated ERK1/2 (p-ERK1/2), JNK (p-JNK), P38 (p-P38). Data represent means ± SEM of p-ERK1/2, p-JNK, p-P38 density, normalized to β-actin values (n = 3). *p < 0.05, **p < 0.01 compared with control, 0 h. (E,F) Double immunofluorescence staining of GFAP (red) with p-JNK (green) and p-ERK1/2 (green) in brain sections of of WT and KO pMCAO mice. (E) Co-staining of GFAP with p-JNK in brain sections of WT and KO pMCAO (6 h) mice; (F) Co-staining of GFAP with p-ERK in brain sections of WT and KO pMCAO (1 h) mice. Nuclei were stained in blue with DAPI. Scale bar: 25 μm. WT, wild type; KO, knockout.