Figure 2.

Galectin-1 expression on IFNγ- and IL-10-producing CD4⁺ T cells during established infection. IFNγ and IL-10 production by CD4⁺ T cells from C57BL/6 mice (n = 5) infected with Leishmania donovani (day 56 p.i.) was assessed by flow cytometry. The gating strategy used to identify cytokine-producing CD4⁺ T cells is shown (A). The frequency and number of galectin-1-positive cytokine-producing cells, as well as the relative expression of galectin-1 determined by mean fluorescent intensity (MFI), on these cells in the liver (B) and spleen (C) were measured, as indicated. These data represent one of two separate experiments (**p < 0.01; Mann–Whitney test between IL-10⁻ IFNγ⁺ and IL-10⁺ IFNγ⁺ CD4⁺ T cells only is shown).