Figure 4.

Galectin-1 expression on splenic antigen-presenting cells during established infection. Galectin-1 expression on antigen-presenting cells from C57BL/6 mice (n = 5) infected with Leishmania donovani (day 56 p.i.) was assessed by flow cytometry and compared with naïve control animals. The gating strategy used to identify antigen-presenting cells in the spleen was the same as shown in Figure 3A. The frequency and number of galectin-1-positive cells, as well as the relative expression of galectin-1 determined by mean fluorescent intensity (MFI) from histograms, were measured, as indicated. Dotted histograms reflect isotype control antibody staining, dark gray histograms reflect cells from naïve animals, and white histograms reflect cells from infected animals. These data represent one of two separate experiments (**p < 0.01; Mann–Whitney test, relative to naïve).